一种基于聚四氟乙烯的系统，用于将多向电压施加到脂质双层作为膜蛋白的新平台
A Teflon-based system for applying multidirectional voltages to lipid bilayers as a novel platform for membrane proteins
摘要：
人工双层类脂膜（BLM），以及膜片钳膜，经常用于膜蛋白的功能分析。在这两种方法中，膜的电特性仅由一个参数表征，即跨膜电位。本文报道了一种新型的BLM系统的构建，该系统除了传统的跨膜方向外，还可以在横向方向上控制膜电压。在聚四氟乙烯薄膜上制作了一个微孔，并在微孔周围蒸镀了钛电极。在孔中可再现地形成BLM，而不受电极存在的影响。横向电压的应用不会引起BLM的电特性，如基线电流，跨膜电阻和跨膜电容的显着变化。相反，横向电压明显影响生物离子通道的活动，这表明横向电压可能是一个有用的参数，用于分析通道活动。目前的聚四氟乙烯为基础的系统，其中多向电压可以施加到BLM代表一个很有前途的平台，用于分析膜蛋白的基本功能特性。
英文摘要：
Artificial bilayer lipid membranes (BLMs), along with patch-clamped membranes, are frequently used for functional analyses of membrane proteins. In both methods, the electric properties of membranes are characterized by only one parameter, namely, transmembrane potential. Here the construction of a novel BLM system was reported, in which membrane voltages can be controlled in a lateral direction in addition to conventional transmembrane direction. A microaperture was fabricated in a Teflon film and Ti electrodes were evaporated around the aperture. BLMs were reproducibly formed in the aperture without being affected by the presence of the electrodes. The application of a lateral voltage induced no significant changes in the electric properties of the BLMs, such as baseline current, transmembrane resistance, and transmembrane capacitance. In contrast, lateral voltages clearly affected the activities of biological ion channels, suggesting that the lateral voltage might be a useful parameter for analyzing channel activities. The present Teflon-based system in which multidirectional voltages can be applied to BLMs represent a promising platform for the analysis of underlying functional properties of membrane proteins.
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