一种用于无标记纳米显微镜的超冷凝器
A Super-Condenser for Labelfree Nanoscopy
摘要：
无标记纳米显微镜包括使用可见波长的分辨率在100 nm范围内的光学成像。在这里，我们提出了一种无标签的纳米显微镜方法，结合傅立叶重叠关联与波导显微镜实现“超级聚光器”，其特征在于最大程度地倾斜相干暗场照明与人工拉伸波矢量，由于大的折射率的就业硅$_3$ N $_4$波导材料。我们通过锥形单模波导在尺寸为400 $\mathrmμ$ m $^2$的成像区域上产生傅立叶重叠关联成像所需的相干平面波照明，并双重处理傅立叶重叠关联相位恢复算法的重叠约束：首先通过波导芯片布局的复用输入结构依次改变照明波矢量的方向性，通过在可见光谱上逐步增加照明光波长来缩短波矢量模量。我们通过计算机和体外实验验证了该方法，并提供了有关基本图像形成理论和重建算法的详细信息。
英文摘要：
Labelfree nanoscopy encompasses optical imaging with resolution in the 100 nm range using visible wavelengths. Here, we present a labelfree nanoscopy method that combines Fourier ptychography with waveguide microscopy to realize a 'super-condenser' featuring maximally inclined coherent darkfield illumination with artificially stretched wave vectors due to large refractive indices of the employed Si$_3$N$_4$ waveguide material. We produce the required coherent plane wave illumination for Fourier ptychography over imaging areas 400 $\mathrmμ$m$^2$ in size via adiabatically tapered single-mode waveguides and tackle the overlap constraints of the Fourier ptychography phase retrieval algorithm two-fold: firstly, the directionality of the illumination wave vector is changed sequentially via a multiplexed input structure of the waveguide chip layout and secondly, the wave vector modulus is shortend via step-wise increases of the illumination light wavelength over the visible spectrum. We validate the method via in silico and in vitro experiments and provide details on the underlying image formation theory as well as the reconstruction algorithm.
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