用于低成本无透镜全息细胞成像的3D可打印便携式开源平台
3D-printable portable open-source platform for low-cost lens-less holographic cellular imaging
摘要：
数字全息显微镜是一种新兴的潜在的低成本替代传统的光学显微镜的微物体成像在地球上，水下和空间。然而，立即访问微米级物体需要一个平衡良好的系统设计和复杂的重建算法，这是商业上可获得的，但不能以成本效益的方式访问。在这里，我们提出了一个开源的实现无镜头数字内联全息显微镜平台，基于现成的光学，电子和机械组件，成本不到190美元。它采用了一个蓝光半导体激光拾取器或发光二极管，一个针孔，一个由3个部分组成的3D打印外壳，以及一个单板便携式计算机和相机，并采用了开源的Fresnel-Kirchhoff例程。实验结果表明，激光头的空间分辨率为1.55 μ m，发光二极管光源的空间分辨率为3.91 μ m。外壳和机械部件都是3D打印的。打印机和重建软件源代码都是开放的。这种轻型显微镜可以对直径为6.5 μ m的无标记微球、直径约为8 μ m的人类红细胞以及50 μ m大小的快速生长植物烟草-BY-2悬浮细胞进行成像。通过涉及标准化测试目标的成像对比度量化来验证成像能力。所展示的3D可打印便携式开源平台代表了一种完全开放的设计，低成本的模块化和多功能的成像解决方案，可用于世界上高资源和低资源地区。
英文摘要：
Digital holographic microscopy is an emerging potentially low-cost alternative to conventional light microscopy for micro-object imaging on earth, underwater and in space. Immediate access to micron-scale objects however requires a well-balanced system design and sophisticated reconstruction algorithms, that are commercially available, however not accessible cost-efficiently. Here, we present an open-source implementation of a lens-less digital inline holographic microscope platform, based on off-the-shelf optical, electronic and mechanical components, costing less than $ 190. It employs a Blu-Ray semiconductor-laser-pickup or a light-emitting-diode, a pinhole, a 3D-printed housing consisting of 3 parts and a single-board portable computer and camera with an open-source implementation of the Fresnel-Kirchhoff routine. We demonstrate 1.55 μm spatial resolution by laser-pickup and 3.91 μm by the light-emitting-diode source. The housing and mechanical components are 3D printed. Both printer and reconstruction software source codes are open. The light-weight microscope allows to image label-free micro-spheres of 6.5 μm diameter, human red-blood-cells of about 8 μm diameter as well as fast-growing plant Nicotiana-tabacum-BY-2 suspension cells with 50 μm sizes. The imaging capability is validated by imaging-contrast quantification involving a standardized test target. The presented 3D-printable portable open-source platform represents a fully-open design, low-cost modular and versatile imaging-solution for use in high- and low-resource areas of the world.
机构、总结、中文摘要均由AI分析而来，可能存在错误，请以论文正文为准。
