利用全切片成像技术对Posidonia Oceanica海草通气组织的初步研究
Characterization of Posidonia Oceanica Seagrass Aerenchyma through Whole Slide Imaging: A Pilot Study
摘要：
表征海草的组织形态和解剖结构对于预测其声学行为至关重要。在这项试点研究中，我们使用组织学技术和整体幻灯片成像（WSI）描述的组成和拓扑结构的通气组织的整个叶片在自动的方式相结合的优势，X射线显微断层扫描和光学显微镜。石蜡块的制备方式是，切片机切片包含沿刀片全长分布的任意数量的横截面。石蜡块中的样品组织与整个载玻片图像分析相结合，可实现高通量数据提取和沿整个叶片长度的详尽表征。该工作的核心是图像处理算法，可以识别纤维束，表皮，叶肉和维管系统的细胞和空气陷窝（或空隙）。一组特定的功能开发，以充分描述标准描述符失败的细胞和空隙的凸度。这些特征仔细检查对象边界的局部曲率，以允许通过机器学习准确区分空隙和单元。该算法允许重建与组织密度、可压缩性和刚度相关的细胞和细胞膜特征。然后从高分辨率切片中提取不同细胞类型和气体空间的尺寸分布、总生物量和总空隙体积分数，以提供沿着叶子从其基部到顶端的组织的完整表征。
英文摘要：
Characterizing the tissue morphology and anatomy of seagrasses is essential to predicting their acoustic behavior. In this pilot study, we use histology techniques and whole slide imaging (WSI) to describe the composition and topology of the aerenchyma of an entire leaf blade in an automatic way combining the advantages of X-ray microtomography and optical microscopy. Paraffin blocks are prepared in such a way that microtome slices contain an arbitrarily large number of cross sections distributed along the full length of a blade. The sample organization in the paraffin block coupled with whole slide image analysis allows high throughput data extraction and an exhaustive characterization along the whole blade length. The core of the work are image processing algorithms that can identify cells and air lacunae (or void) from fiber strand, epidermis, mesophyll and vascular system. A set of specific features is developed to adequately describe the convexity of cells and voids where standard descriptors fail. The features scrutinize the local curvature of the object borders to allow an accurate discrimination between void and cell through machine learning. The algorithm allows to reconstruct the cells and cell membrane features that are relevant to tissue density, compressibility and rigidity. Size distribution of the different cell types and gas spaces, total biomass and total void volume fraction are then extracted from the high resolution slices to provide a complete characterization of the tissue along the leave from its base to the apex.
机构、总结、中文摘要均由AI分析而来，可能存在错误，请以论文正文为准。
