线性扫描ATR-FTIR用于微流控通道中假单胞菌生物膜的化学作图和高通量研究
Linear scanning ATR-FTIR for chemical mapping and high-throughput studies of Pseudomonas sp. biofilms in microfluidic channels
摘要：
介绍了一种用于傅里叶变换红外（FTIR）光谱分析的全自动线性扫描衰减全反射（ATR）附件。该方法是基于一个多反弹ATR晶体相对于一个固定的红外光束的准确位移。为了确保准确定位并提供第二种样品表征模式，将定制显微镜集成到系统和计算机化工作流程中。定制软件包括自动化控制和测量程序，具有简单的用户界面，用于选择参数和监控实验进度。这一经济高效的模块化系统可在任何具有标准样品室的研究级光谱仪上实施，用于新的生物分析化学研究。该系统进行了验证和优化，用于与含有生长假单胞菌属细菌生物膜的微流体流动池。扫描定位精度、测量空间分辨率和微通道尺寸之间的互补性为实时控制环境参数和最少的人工劳动的并行生物测定铺平了道路。通过相对于光束路径旋转通道取向，该系统还可以用于沿着通道长度采集线性生化图和拼接的显微镜图像。
英文摘要：
A fully automated linear scanning attenuated total reflection (ATR) accessory is presented for Fourier transform infrared (FTIR) spectroscopy. The approach is based on the accurate displacement of a multi-bounce ATR crystal relative to a stationary infrared beam. To ensure accurate positioning and to provide a second sample characterization mode, a custom-built microscope was integrated into the system and the computerized work flow. Custom software includes automated control and measurement routines with a straightforward user interface for selecting parameters and monitoring experimental progress. This cost-effective modular system can be implemented on any research-grade spectrometer with a standard sample compartment for new bioanalytical chemistry studies. The system was validated and optimized for use with microfluidic flow cells containing growing Pseudomonas sp. bacterial biofilms. The complementarity among the scan positioning accuracy, measurement spatial resolution and the microchannel dimensions paves the way for parallel biological assays with real-time control over environmental parameters and minimal manual labor. By rotating the channel orientation relative to the beam path, the system could also be used for acquisition of linear biochemical maps and stitched microscope images along the channel length.
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