单细胞去顶：探测天然膜的拓扑结构和纳米力学
Single-cell unroofing: probing topology and nanomechanics of native membranes
摘要：
细胞膜将细胞内部与外部环境隔开。它们由各种脂质构成;它们的组成决定了膜蛋白的动态并影响细胞的适应能力。尽管模型膜的研究可以理解脂质之间的相互作用和整体力学，但对天然膜中的这些性质知之甚少。为了结合天然膜的拓扑结构和纳米力学分析，我设计了一种方法来研究从各种单细胞中分离的质膜。选择五种细胞类型并进行测试，揭示了20%的膜厚度变化。我探测了隔离膜对水的阻力，发现它们的线张力和扩散压力。这些结果表明，从神经元分离的膜比脑癌细胞膜更硬，扩散性更低。这种方法提供了直接的定量见解天然细胞膜的力学。
英文摘要：
Cell membranes separate the cell interior from the external environment. They are constituted by a variety of lipids; their composition determines the dynamics of membrane proteins and affects the ability of the cells to adapt. Even though the study of model membranes allows to understand the interactions among lipids and the overall mechanics, little is known about these properties in native membranes. To combine topology and nanomechanics analysis of native membranes, I designed a method to investigate the plasma membranes isolated from a variety of single cells. Five cell types were chosen and tested, revealing 20\% variation in membrane thickness. I probed the resistance of the isolated membranes to indent, finding their line tension and spreading pressure. These results show that membranes isolated from neurons are stiffer and less diffusive than brain cancer cell membranes. This method gives direct quantitative insights on the mechanics of native cell membranes.
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