分子和结构生物学中的分析光散射方法：实验方面和结果
Analytical light scattering methods in molecular and structural biology: Experimental aspects and results
摘要：
非侵入性光散射方法提供生物大分子（即蛋白质、核酸以及由它们组成的组装体和较大实体）的数据，这些数据与尺寸排阻色谱法、凝胶电泳、分析超离心和质谱法的数据互补。静态光散射测量对于确定大分子的质量和监测聚集现象是有用的。动态光散射测量适用于质量控制和评估样品均匀性，以确定粒度，检查物理和化学处理的效果，探测配体的结合，并研究大分子之间的相互作用。
英文摘要：
Non-invasive light scattering methods provide data on biological macromolecules (i.e. proteins, nucleic acids, as well as assemblies and larger entities composed of them) that are complementary with those of size exclusion chromatography, gel electrophoresis, analytical ultracentrifugation and mass spectrometry methods. Static light scattering measurements are useful to determine the mass of macromolecules and to monitor aggregation phenomena. Dynamic light scattering measurements are suitable for the quality control and to assess sample homogeneity, to determine particle size, examine the effect of physical and chemical treatments, probe the binding of ligands, and study interactions between macromolecules.
机构、总结、中文摘要均由AI分析而来，可能存在错误，请以论文正文为准。
