溶液中的扭结DNA：核小体定位序列的小角散射研究
Kinky DNA in solution: Small angle scattering study of a nucleosome positioning sequence
摘要：
DNA是一种灵活的分子，但其灵活性的程度受到争议。普遍接受的$l_p \approx 500\,$ DNA的持续长度与最近对短链DNA的研究不一致，后者显示出更大的灵活性，但没有探测其起源。我们已经进行了X射线和中子小角散射的短DNA序列含有一个强大的核小体定位元素，并分析结果，使用修改后的Kratky Porod模型，以确定可能的构象。我们的研究结果支持了Crick和Klug在1975年提出的一个假设，即溶液中的某些DNA序列可能具有尖锐的扭结，从而可能解决这种差异。我们的结论是支持测量辐射损伤的样品，单链断裂导致增加的灵活性和数据分析从另一个序列，它没有扭结，但我们的方法可以检测到局部增强的灵活性，由于$AT$域。
英文摘要：
DNA is a flexible molecule, but the degree of its flexibility is subject to debate. The commonly-accepted persistence length of $l_p \approx 500\,$Å is inconsistent with recent studies on short-chain DNA that show much greater flexibility but do not probe its origin. We have performed X-ray and neutron small-angle scattering on a short DNA sequence containing a strong nucleosome positioning element, and analyzed the results using a modified Kratky-Porod model to determine possible conformations. Our results support a hypothesis from Crick and Klug in 1975 that some DNA sequences in solution can have sharp kinks, potentially resolving the discrepancy. Our conclusions are supported by measurements on a radiation-damaged sample, where single-strand breaks lead to increased flexibility and by an analysis of data from another sequence, which does not have kinks, but where our method can detect a locally enhanced flexibility due to an $AT$-domain.
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