用于三维细胞培养的冷冻铸造微脊胶原支架的局部成纤维
Topotactic fibrillogenesis of freeze-casted microridged collagen scaffolds for 3D cell culture
摘要：
I型胶原是细胞外基质（ECM）的主要成分。在体外，在物理化学条件的窄窗口下，I型胶原蛋白自组装以形成复杂的超分子结构，这让人想起在天然ECM中发现的那些。目前，基于胶原蛋白的生物材料的一个主要挑战是将微妙的胶原蛋白原纤维形成事件与非变性条件下的受控成形过程结合起来。在这项工作中，冰模板的方法，促进大孔整料的胶原蛋白的结构。代替常见的溶剂去除程序，一个新的拓扑结构的转换方法产生自组装有序的纤维材料的实施。这些仅含胶原蛋白的非交联支架在湿态下表现出不寻常的机械性能。在冰图案微脊特征的帮助下，正常人真皮成纤维细胞和C2C12鼠成肌细胞成功迁移并在所得3D仿生胶原支架内形成高度对齐的群体。这些结果为开发从分子到大孔的各种组织水平有序的新组织工程支架开辟了一条新途径，并且对于需要大规模3D细胞对齐的生物医学应用特别感兴趣，例如肌肉或神经重建。
英文摘要：
Type I collagen is the main component of the extra-cellular matrix (ECM). In vitro, under a narrow window of physico-chemical conditions, type I collagen self-assembles to form complex supramolecular architectures reminiscent of those found in native ECM. Presently, a major challenge in collagen-based biomaterials is to couple the delicate collagen fibrillogenesis events with a controlled shaping process in non-denaturating conditions. In this work an ice-templating approach promoting the structuration of collagen into macroporous monoliths is used. Instead of common solvent removal procedures, a new topotactic conversion approach yielding self-assembled ordered fibrous materials is implemented. These collagen-only, non-cross-linked scaffolds exhibit uncommon mechanical properties in the wet state. With the help of the ice-patterned micro-ridge features, Normal Human Dermal Fibroblasts and C2C12 murine myoblasts successfully migrate and form highly-aligned populations within the resulting 3D biomimetic collagen scaffolds. These results open a new pathway to the development of new tissue engineering scaffolds ordered across various organization levels from the molecule to the macropore, and are of particular interest for biomedical applications where large scale 3D cell alignment is needed such as for muscular or nerve reconstruction.
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