使用彩色空间光干涉显微镜（cSLIM）对染色组织进行定量组织学检查
Quantitative Histopathology of Stained Tissues using Color Spatial Light Interference Microscopy (cSLIM)
摘要：
临床上的组织活检评估需要定量的疾病标志物用于诊断，最重要的是，预后。在这些新技术中，定量相位成像（QPI）已经证明了组织病理学的前景，因为它通过折射率揭示了内在的组织纳米结构。然而，绝大多数过去的QPI研究依赖于成像未染色的组织，这破坏了既定的标本处理。在这里，我们提出了彩色空间光干涉显微镜（cSLIM）作为一种新的整体载玻片成像方式，进行干涉成像与彩色探测器阵列。因此，cSLIM在单次扫描中产生病理学家熟悉的内在组织相位图和标准彩色明场图像。我们对196例乳腺癌患者的研究结果表明，cSLIM不仅可以提供诊断信息，还可以从肿瘤微环境中胶原纤维的排列中提供预后信息。通过简单的数学归一化校正染色对组织相位图的影响。这些特征可能会减少新cSLIM技术的临床转化障碍。
英文摘要：
Tissue biopsy evaluation in the clinic is in need of quantitative disease markers for diagnosis and, most importantly, prognosis. Among the new technologies, quantitative phase imaging (QPI) has demonstrated promise for histopathology because it reveals intrinsic tissue nanoarchitecture through the refractive index. However, a vast majority of past QPI investigations have relied on imaging unstained tissues, which disrupts the established specimen processing. Here we present color spatial light interference microscopy (cSLIM) as a new whole slide imaging modality that performs interferometric imaging with a color detector array. As a result, cSLIM yields in a single scan both the intrinsic tissue phase map and the standard color bright-field image, familiar to the pathologist. Our results on 196 breast cancer patients indicate that cSLIM can provide not only diagnostic but also prognostic information from the alignment of collagen fibers in the tumor microenvironment. The effects of staining on the tissue phase maps were corrected by a simple mathematical normalization. These characteristics are likely to reduce barriers to clinical translation for the new cSLIM technology.
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