远红外荧光蛋白与蚕丝杂交的可见光光敏化
Visible Light-Activated Photosensitization of Hybridization of Far-red Fluorescent Protein and Silk
摘要：
荧光蛋白通常导致光毒性和细胞毒性，特别是因为一些红色荧光蛋白（RFP）产生和释放活性氧（ROS）。ROS的光生成被认为是细胞成像中的有害副作用，或者被主动用于消融癌组织。作为古老的纺织品或生物材料，蚕产生的蚕丝可以直接用作织物或加工成材料和结构，以承载其他功能纳米材料。我们报告说，远红荧光蛋白（mKate2）与丝绸的转基因融合提供了一个光敏剂杂交平台，光诱导控制活性氧。利用绿色（可见）光激活，天然和再生的mKate 2丝可以以与可见光驱动的等离子体光催化相当的方式产生和释放超氧化物和单线态氧。因此，mKate2在丝中的基因表达提供了立即可开发和可扩展的光催化剂样生物材料。我们进一步设想，mKate2丝可以潜在地排除与外来半导体光催化纳米材料相关的危险问题。
英文摘要：
Fluorescent proteins often result in phototoxicity and cytotoxicity, in particular because some red fluorescent proteins (RFP) produce and release reactive oxygen species (ROS). The photogeneration of ROS is considered as a detrimental side effect in cellular imaging or is proactively utilized for ablating cancerous tissue. As ancient textiles or biomaterials, silk produced by silkworms can directly be used as fabrics or be processed into materials and structures to host other functional nanomaterials. We report that transgenic fusion of far-red fluorescent protein (mKate2) with silk provides a photosensitizer hybridization platform for photoinducible control of ROS. Taking advantage of green (visible) light activation, native and regenerated mKate2 silk can produce and release superoxide and singlet oxygen, in a comparable manner of visible light-driven plasmonic photocatalysis. Thus, the genetic expression of mKate2 in silk offers immediately exploitable and scalable photocatalyst-like biomaterials. We further envision that mKate2 silk could potentially rule out hazardous concerns associated with foreign semiconductor photocatalytic nanomaterials.
机构、总结、中文摘要均由AI分析而来，可能存在错误，请以论文正文为准。
