利用RAPD标记分析4种茄子的遗传多样性〈动〉和基因组间的亲缘关系
Analysis of genetic diversity and genome relationships of four eggplant species (Solanum melongena L) using RAPD markers
摘要：
茄（Solanum melongena）是茄科植物中的一种，在印度尼西亚种植并广泛传播，并被社区广泛使用。本研究采用RAPD（Random Amplified Polymorphic DNA）技术对印尼4个地方茄子品种Leuca、Tekokak、Gelatik和Kopek的遗传多样性〈动〉性<动>进行了分析。样品来自印度尼西亚茂物农业技术评估研究所（BPTP）。数据观测结果以茄属植物DNA图谱的形式进行了连续性和定量分析。用4个引物（OPF 01、OPF 02、OPF 03和OPF 04）共扩增出30条DNA带，其中多态性28条，单态2条。所使用的引物能够扩增所有四个茄子样品。PCR-RAPD可视化结果显示300-1500 bp的条带。聚类分析结果表明，存在A、B、C三个聚类。簇A（系数等于49%）由明胶组成，簇B（65%平衡系数）由TPU（Kopek）和TK（Tekokak）组成，簇C（55%平衡系数）由LC（Leunca）组成。这些结果表明，TK（Tekokak）和TPU（Kopek）样品中存在最接近性。
英文摘要：
Solanum melongena (eggplant) is one of the diversity of the Solanum family which is grown and widely spread in Indonesia and widely used by the community. This research explored the genetic diversity of four local Indonesian eggplant species namely leuca, tekokak, gelatik and kopek by using RAPD (Random Amplified Polymorphic DNA). The samples were obtained from Agricultural Technology Assessment Institute (BPTP) Bogor, Indonesia. The result of data observation was in the form of Solanum melongena plants DNA profile analyzed descriptively and quantitatively. 30 DNA bands (28 polymorphic and 2 monomorphic) were successfully scored by using four primers (OPF01, OPF02, OPF03 and OPF04). The Primers were used able to amplify all of the four eggplant samples. The result of PCR-RAPD visualization produces bands of 300-1500 bp. The result of cluster analysis showed the existence of three clusters (A, B, and C). Cluster A (coefficient of equal to 49%) consisted of a gelatik, cluster B (coefficient of 65% equilibrium) consisted of TPU (Kopek) and TK (Tekokak), and cluster C (55% equilibrium coefficient) consisted of LC (Leunca). These results indicated that the closest proximity is found in samples of TK (Tekokak) and TPU (Kopek)
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