活细胞成像系统的模拟揭示了合作结合测量中隐藏的不确定性
Simulation of live-cell imaging system reveals hidden uncertainties in cooperative binding measurements
摘要：
我们提出了一种计算方法来定量评估系统的不确定性，所产生的不可检测的来源，在生物测量使用活细胞成像技术。然后，我们证明了这种方法在测量分子结合网络的生物协同性：特别是，配体分子结合细胞表面受体蛋白。我们的研究结果表明，如何非统计的不确定性导致无效的识别测得的协同性。通过这种计算方案，生物学解释可以更客观地评估和理解下一个特定的实验配置的利益。
英文摘要：
We propose a computational method to quantitatively evaluate the systematic uncertainties that arise from undetectable sources in biological measurements using live-cell imaging techniques. We then demonstrate this method in measuring biological cooperativity of molecular binding networks: in particular, ligand molecules binding to cell surface receptor proteins. Our results show how the non-statistical uncertainties lead to invalid identification of the measured cooperativity. Through this computational scheme, the biological interpretation can be more objectively evaluated and understood under a specific experimental configuration of interest.
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