AMPA、NMDA和GABAA受体介导的体外皮层神经网络爆发动力学
AMPA, NMDA and GABAA receptor mediated network burst dynamics in cortical cultures in vitro
摘要：
本工作研究了兴奋性AMPA、NMDA和抑制性GABAA受体介导的离体新生大鼠皮层神经元网络的动态变化。在不同的药理学条件下，用59个平面电极同时记录细胞外网络范围的活性。我们分析了基线和AMPA受体（AMPA-R）或NMDA受体（NMDA-R）驱动的活动之间，以及后一种状态和去抑制活动之间的整体网络活动和网络范围的爆发频率的变化。此外，我们还研究了神经网络修饰爆发的时空结构和网络爆发过程中电极的募集。我们的研究结果表明，AMPA-R和NMDA-R受体在网络动力学中具有明显不同的作用。AMPA-R在发起网络范围的突发方面具有更大的优势。因此，NMDA-R保持已经启动的活动。GABAA受体（GABAA-Rs）在爆发期间比NMDA-R驱动的活性更强烈地抑制AMPA-R驱动的网络活性。
英文摘要：
In this work we study the excitatory AMPA, and NMDA, and inhibitory GABAA receptor mediated dynamical changes in neuronal networks of neonatal rat cortex in vitro. Extracellular network-wide activity was recorded with 59 planar electrodes simultaneously under different pharmacological conditions. We analyzed the changes of overall network activity and network-wide burst frequency between baseline and AMPA receptor (AMPA-R) or NMDA receptor (NMDA-R) driven activity, as well as between the latter states and disinhibited activity. Additionally, spatiotemporal structures of pharmacologically modified bursts and recruitment of electrodes during the network bursts were studied. Our results show that AMPA-R and NMDA-R receptors have clearly distinct roles in network dynamics. AMPA-Rs are in greater charge to initiate network wide bursts. Therefore NMDA-Rs maintain the already initiated activity. GABAA receptors (GABAA-Rs) inhibit AMPA-R driven network activity more strongly than NMDA-R driven activity during the bursts.
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