SPION细胞摄取机制的体外研究
The cellular uptake mechanism of SPIONs: an in-vitro study
摘要：
超顺磁性氧化铁纳米粒子（SPION）具有局部热疗、磁共振成像、靶向性和药物释放等特性，在生物学研究中得到了广泛应用。细胞毒性的体外研究和对细胞摄取的特定途径的理解将能够操纵SPION用于靶向治疗的最佳细胞摄取的条件。本研究的目的是确定C6胶质瘤细胞摄取SPION的内吞途径。将细胞与不同浓度的药理学抑制剂预孵育，然后暴露于SPION几个小时。内吞的颗粒进行定位和定量估计使用Perl的或普鲁士蓝反应。当与抑制剂一起孵育时，SPION的摄取显著减少，表明纳米颗粒的摄取受到抑制。发现SPION摄取的这种减少取决于抑制剂的浓度以及抑制剂的性质。通过选择不同抑制剂的系统研究，可以将数据缩小到可能参与SPION摄取的最终途径。目前的初步调查，预计将提供洞察力的测试与补充技术的可能机制。
英文摘要：
The Superparamagnetic Iron Oxide Nanoparticles (SPIONs) of sizes ranging from 10-50 nm are being used in a large number of biological studies because of their peculiar characteristics of inducing local hyperthermia, MR imaging, specific targeting and drug delivery. An in-vitro study of the cytotoxicity and an understanding of the specific pathway of cellular uptake will enable manipulation of conditions for optimal cellular uptake of SPIONs for targeted therapy. The objective of the present study was to identify the endocytotic pathway through which the SPIONs are taken up by C6 glioma cells. The cells were pre-incubated with different concentrations of pharmacological inhibitors and then exposed to SPIONs for a few hours. The endocytosed particles were localized and quantitatively estimated using Perl's or Prussian Blue reaction. There was significant reduction in the uptake of SPIONs when incubated with the inhibitor indicating the uptake of nanoparticles is being inhibited. This reduction in SPION uptake was found to be dependent on the concentration of the inhibitors and also the nature of the inhibitors. By a systematic study of choosing various inhibitors, the data can be narrowed down to the final pathway that may be involved in the SPION uptake. The present preliminary investigation is expected to provide insight of testing the possible mechanisms with complementary techniques.
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