具有绕倾斜轴的光流旋转的单细胞衍射层析成像
Single-cell diffraction tomography with optofluidic rotation about a tilted axis
摘要：
光学衍射层析成像（Optical Diffraction Tomography，ODT）是一种不需要任何标记物就可以测量活细胞内三维（3D）折射率分布的层析成像技术。原理上，ODT可以被看作是光学投影层析成像的推广，其等同于计算机层析成像（CT）。这两种光学层析成像技术都需要在多个角度测量细胞的投影相位图像。然而，这两种技术测量后3D折射率分布的重建不同。已知的是，ODT产生比投影层析成像更好的结果，因为它考虑了由于样品的折射率结构引起的成像光的衍射。在这里，我们将ODT应用于微流控芯片中的生物细胞，该微流控芯片结合了光捕获和微流控流动以实现光流控单细胞旋转。特别是，我们解决的问题时，被困的细胞不旋转轴垂直于成像平面，而是围绕一个任意倾斜的轴。在本文中，我们表明，考虑到这样一个倾斜的旋转轴在重建过程中的三维重建可以得到改善。
英文摘要：
Optical diffraction tomography (ODT) is a tomographic technique that can be used to measure the three-dimensional (3D) refractive index distribution within living cells without the requirement of any marker. In principle, ODT can be regarded as a generalization of optical projection tomography which is equivalent to computerized tomography (CT). Both optical tomographic techniques require projection-phase images of cells measured at multiple angles. However, the reconstruction of the 3D refractive index distribution post-measurement differs for the two techniques. It is known that ODT yields better results than projection tomography, because it takes into account diffraction of the imaging light due to the refractive index structure of the sample. Here, we apply ODT to biological cells in a microfluidic chip which combines optical trapping and microfluidic flow to achieve an optofluidic single-cell rotation. In particular, we address the problem that arises when the trapped cell is not rotating about an axis perpendicular to the imaging plane, but instead about an arbitrarily tilted axis. In this paper we show that the 3D reconstruction can be improved by taking into account such a tilted rotational axis in the reconstruction process.
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