刚地弓形虫钙动力学的计算运动跟踪
Computational Motility Tracking of Calcium Dynamics in Toxoplasma gondii
摘要：
弓形虫是弓形虫病的病原体，是世界上最常见的寄生虫之一。对于一个成功的裂解周期，T.弓形虫必须穿过生物屏障才能侵入宿主细胞，因此，运动性对其毒力至关重要。钙信号传导受细胞内钙离子浓度波动的控制，在整个生命过程中被普遍利用，并调节许多细胞过程，包括T细胞的刺激。弓形虫的毒力因子如运动性。因此，细胞内钙的增加，称为钙振荡，作为一种手段，以联系和量化的细胞内信号传导过程，导致T。弓形虫运动和侵袭。在这里，我们描述了我们的工作提取，量化和建模运动模式的T。在加入药理学药物和/或细胞外钙之前和之后的弓形虫。我们展示了一个计算管道，包括一个强大的跟踪系统，使用光流和密集的轨迹特征提取T。弓形虫运动模式使用这个管道，我们能够跟踪响应细胞外寄生虫胞质Ca 2+通量的弓形虫运动性的变化。这使我们能够研究通过细胞内Ca 2+储存和/或细胞外Ca 2+进入释放的Ca 2+信号传导如何与运动模式相关，这是开发T.弓形虫毒力
英文摘要：
Toxoplasma gondii is the causative agent responsible for toxoplasmosis and serves as one of the most common parasites in the world. For a successful lytic cycle, T. gondii must traverse biological barriers in order to invade host cells, and as such, motility is critical for its virulence. Calcium signaling, governed by fluctuations in cytosolic calcium (Ca2+) concentrations, is utilized universally across life and regulates many cellular processes, including the stimulation of T. gondii virulence factors such as motility. Therefore, increases in cytosolic calcium, called calcium oscillations, serve as a means to link and quantify the intracellular signaling processes that lead to T. gondii motility and invasion. Here, we describe our work extracting, quantifying and modeling motility patterns of T. gondii before and after the addition of pharmacological drugs and/or extracellular calcium. We demonstrate a computational pipeline including a robust tracking system using optical flow and dense trajectory features to extract T. gondii motility patterns. Using this pipeline, we were able to track changes in T.gondii motility in response to cytosolic Ca2+ fluxes in extracellular parasites. This allows us to study how Ca2+ signaling via release from intracellular Ca2+ stores and/or from extracellular Ca2+ entry relates to motility patterns, a crucial first step in developing countermeasures for T. gondii virulence.
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