微分动态显微术中有限尺寸图像伪影的校正
Correcting artifacts from finite image size in Differential Dynamic Microscopy
摘要:
差分动态显微镜(DDM)分析传统的真实空间显微镜图像,以类似于光散射的方式提取样品动态信息,通过将序列中的每个图像分解为傅立叶模式,并评估其时间相关性。DDM已应用于许多软物质和胶体体系。然而,观察到的物体移出显微镜捕获的视场,与所获取图像的边缘相交,可能会在随后的分析中引入虚假但显著的误差。在这里,我们表明,应用程序的空间窗口过滤器的图像序列之前,他们进入标准DDM分析可以大大减少这些文物。此外,窗口可以显着增加DDM探测的波矢量的可访问范围,并可能进一步产生意想不到的信息,如胶体悬浮液的尺寸多分散性。
英文摘要:
Differential Dynamic Microscopy (DDM) analyzes traditional real-space microscope images to extract information on sample dynamics in a way akin to light scattering, by decomposing each image in a sequence into Fourier modes, and evaluating their time correlation properties. DDM has been applied in a number of soft-matter and colloidal systems. However, objects observed to move out of the microscope's captured field of view, intersecting the edges of the acquired images, can introduce spurious but significant errors in the subsequent analysis. Here we show that application of a spatial windowing filter to images in a sequence before they enter the standard DDM analysis can reduce these artifacts substantially. Moreover, windowing can increase significantly the accessible range of wave vectors probed by DDM, and may further yield unexpected information, such as the size polydispersity of a colloidal suspension.
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