界面细菌膜（FBI）：铜绿假单胞菌对流体界面的重塑
Films of Bacteria at Interfaces (FBI): Remodeling of Fluid Interfaces by Pseudomonas aeruginosa
摘要：
流体界面处的细菌承受这些高度不对称环境所特有的物理和化学应力。比较了铜绿假单胞菌PAO 1和PA 14对十六烷-水界面的响应。PAO 1细胞形成细菌、分泌的多糖和蛋白质的弹性膜，而PA 14细胞在不形成弹性膜的情况下主动移动。PAO 1突变体的研究表明，与固体支持的生物膜，弹性界面膜的形成发生在鞭毛，菌毛，或某些多糖的情况下。在转录谱中鉴定的高度诱导的基因包括推定的酶和碳水化合物代谢酶alkB 2的基因;后一种基因在PA 14细胞中不上调。值得注意的是，缺乏alkB 2基因的PAO 1突变体不能形成弹性层。相反，它们形成类似于PA 14形成的活性膜。这些研究结果表明，基因表达改变界面限制，并表明代谢烷烃的能力可能发挥作用，在弹性膜形成在油-水界面。
英文摘要：
Bacteria at fluid interfaces endure physical and chemical stresses unique to these highly asymmetric environments. The responses of Pseudomonas aeruginosa PAO1 and PA14 to a hexadecane-water interface are compared. PAO1 cells form elastic films of bacteria, excreted polysaccharides and proteins, whereas PA14 cells move actively without forming an elastic film. Studies of PAO1 mutants show that, unlike solid-supported biofilms, elastic interfacial film formation occurs in the absence of flagella, pili, or certain polysaccharides. Highly induced genes identified in transcriptional profiling include those for putative enzymes and a carbohydrate metabolism enzyme, alkB2; this latter gene is not upregulated in PA14 cells. Notably, PAO1 mutants lacking the alkB2 gene fail to form an elastic layer. Rather, they form an active film like that formed by PA14. These findings demonstrate that genetic expression is altered by interfacial confinement, and suggest that the ability to metabolize alkanes may play a role in elastic film formation at oil-water interfaces.
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