非接触发光寿命低温测温法在大分子晶体学中的应用
Non-contact luminescence lifetime cryothermometry for macromolecular crystallography
摘要：
一种用于远程，非接触，原位监测蛋白质晶体温度的新技术已经开发用于钻石光源的新I23光束线，该光源是一种专用于长波长X射线的大分子晶体学（MX）的设施。温度是从位于非常接近被测样品的微型闪烁传感器（0.05 mm 3）的发光的温度依赖性衰减时间常数得出的。在这项工作中，我们提出了低温发光寿命测温的基本原理，讨论了检测方法的特点，温度传感器的选择，并演示了如何将温度监测系统集成在用于蛋白质晶体可视化的终端站的观察系统中。该测温系统的特点是使用Bi 4Ge 3 O 12（BGO）晶体闪烁体，表现出良好的响应度的衰减时间常数作为温度的函数在很宽的范围内（8-270 K）。对闪烁传感器进行了校准，在束线的主要工作温度范围（30-150 K）内，温度测量的不确定度被评定为± 1.6 K。它已被证明，样品架的温度，使用发光传感器测量，同意与预期值。该技术被应用到不同的样品安装，已在MX实验中使用的I23光束线的热性能进行了验证。底座的厚度被示出对样品底座上的温度分布具有最大的影响。总之，这些测试和研究结果表明，温度测量系统在突出真空MX实验成为结构生物学可靠和不可或缺的工具仍有待解决的挑战方面是有用的
英文摘要：
A novel technique for remote, non-contact, in situ monitoring of the protein crystal temperature has been developed for the new I23 beamline at the Diamond Light Source, a facility dedicated to macromolecular crystallography (MX) with long-wavelength X-rays. The temperature is derived from the temperature-dependant decay time constant of luminescence from a minuscule scintillation sensor (0.05 mm3) located in very close proximity to the sample under test. In this work we present the underlying principle of cryogenic luminescence lifetime thermometry, discuss the features of the detection method, the choice of temperature sensor and demonstrate how the temperature monitoring system was integrated within the viewing system of the end-station used for the visualisation of protein crystals. The thermometry system was characterised using a Bi4Ge3O12 (BGO) crystal scintillator that exhibits good responsivity of the decay time constant as function of temperature over a wide range (8-270 K). The scintillation sensor was calibrated and the uncertainty of the temperature measurements over the primary operation temperature range of the beamline (30-150 K) was assessed to be +-1.6 K. It has been shown that the temperature of the sample holder, measured using the luminescence sensor, agrees well with the expected value. The technique was applied to characterise the thermal performance of different sample mounts that have been used in MX experiments at the I23 beamline. The thickness of the mount is shown to have the greatest impact upon the temperature distribution across the sample mount. Altogether these tests and findings demonstrate the usefulness of the thermometry system in highlighting the challenges that remain to be addressed for the in-vacuum MX experiment to become a reliable and indispensable tool for structural biology
机构、总结、中文摘要均由AI分析而来，可能存在错误，请以论文正文为准。
