来自蛋白质纳米晶体气溶胶束的飞秒X射线衍射
Femtosecond x-ray diffraction from an aerosolized beam of protein nanocrystals
摘要：
我们证明了近原子分辨率的布拉格衍射从雾化的单个颗粒病毒晶体使用X射线自由电子激光。气溶胶喷射器的形式几乎与传统的液体微射流喷嘴相同，但是通过使用氦载气而不是液体，X射线散射背景降低了几个数量级。这种方法提供了一种途径来研究弱扩散或晶格变换信号所产生的小晶体。粒子的高速度特别适合于即将到来的MHz重复率X射线自由电子激光。
英文摘要：
We demonstrate near-atomic-resolution Bragg diffraction from aerosolized single granulovirus crystals using an x-ray free-electron laser. The form of the aerosol injector is nearly identical to conventional liquid-microjet nozzles, but the x-ray-scattering background is reduced by several orders of magnitude by the use of helium carrier gas rather than liquid. This approach provides a route to study the weak diffuse or lattice-transform signal arising from small crystals. The high speed of the particles is particularly well suited to upcoming MHz-repetition-rate x-ray free-electron lasers.
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