ABEL阱中单个纳米粒子的布朗运动
Confining Brownian motion of single nanoparticles in an ABELtrap
摘要：
捕获纳米级物体以观察其长时间的动态行为是一个正在进行的探索。特别是，低于100纳米的透明物体很难捕捉，大多数技术依赖于通过共焦激光焦点的固定或瞬时扩散。我们提出了一种反布朗电动势阱（由A. e. Cohen和W. e. Moerner）以将纳米颗粒和单个FoF1-ATP合酶蛋白保持在溶液中。我们感兴趣的是这种膜结合的旋转马达蛋白，我们使用单分子FRET监测的构象动力学。ABELtrap是一种主动反馈系统，通过施加电场来消除纳米物体的布朗运动。我们展示了如何诱导的动电力限制的纳米粒子和蛋白脂质体的运动的陷阱的中心。
英文摘要：
Trapping nanoscopic objects to observe their dynamic behaviour for extended periods of time is an ongoing quest. Particularly, sub-100nm transparent objects are hard to catch and most techniques rely on immobilisation or transient diffusion through a confocal laser focus. We present an Anti-Brownian ELectrokinetic trap (pioneered by A. E. Cohen and W. E. Moerner) to hold nanoparticles and individual FoF1-ATP synthase proteins in solution. We are interested in the conformational dynamics of this membrane-bound rotary motor protein that we monitor using single-molecule FRET. The ABELtrap is an active feedback system cancelling the nano-object's Brownian motion by applying an electric field. We show how the induced electrokinetic forces confine the motion of nanoparticles and proteoliposomes to the centre of the trap.
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