暴露于UV-B和紫光的未处理和致死处理的大肠杆菌的共焦显微拉曼光谱
Confocal micro-Raman Spectra of untreated and lethally treated Escherichia coli exposed to UV-B and violet light
摘要：
我们报告的变化，在拉曼光谱的活土方ichi大肠杆菌（大肠杆菌），导致暴露于致命的能量密度的300 nm（UV-B）和405 nm（紫）的光子。在第一种情况下，4.13 eV的每光子能量足以破坏细菌中的大部分原子间键，并且观察到拉曼光谱的主要变化，特别是在RNA/DNA区域。该能量与连接磷酸根骨架部分的C-H、N-H和P-O键结合能接近共振，并可能引发拉曼修饰。相比之下，3.06 eV的紫光子能量不足以裂解较强的系统键。在这种情况下所需的大得多的致死通量在共振C-N和C-P键连接的氨基酸/蛋白质基团和脂质峰信号中产生显著变化，但在核苷酸-DNA/RNA特征中的变化要小得多。
英文摘要：
We report on changes in the Raman spectrum of live Escherichi coli (E.coli) that result from exposure to lethal fluences of 300 nm (UV-B) and 405 nm (violet) photons. In the first instance, the energy per photon of 4.13 eV is sufficient to break most of the inter-atomic bonds in the bacterium and major change in the Raman spectrum, particularly in the RNA/DNA regions is observed. This energy is in near resonance with the C-H, N-H, and P-O bond binding energies that interconnect phosphate backbone sections and may initiate the Raman modifications. By contrast, the 3.06 eV violet photon energy is insufficient to cleave the stronger system bonds. The much larger lethal fluence required in this case produces a significant change in the resonant C-N and C-P bond connected amino acid/protein groups and lipid peak signal but much less so in the nucleotide-DNA/RNA signature.
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