药物诱导的视网膜色素上皮细胞形态学演变
Druse-Induced Morphology Evolution in Retinal Pigment Epithelium
摘要：
视网膜色素上皮（retinal pigment epithelium，RPE）是许多视网膜疾病的重要发病部位。视网膜玻璃疣的形成是视网膜变性的特征。我们采用综合实验和建模的方法研究了存在软性玻璃疣时RPE的形态学变化。我们从捐赠的人眼中收集RPE平板图像，并开发1）统计工具来量化图像，2）基于细胞的模型来模拟形态演变。我们比较了三种不同的机制的RPE修复进化，细胞凋亡，细胞融合和扩张，我们的RPE形态发生模型的模拟定量再现人类RPE形态由于玻璃疣的变形，这表明荷包机制足以解释RPE愈合玻璃疣损伤引起的细胞损失。我们发现，组织下的玻璃疣促进细胞死亡的数量远远超过覆盖玻璃疣的细胞数量。组织变形研究使用面积分布，Voronoi域和纹理张量。
英文摘要：
The retinal pigment epithelium (RPE) is a key site of pathogenesis for many retina diseases. The formation of drusen in the retina is characteristic of retinal degeneration. We investigate morphological changes in the RPE in the presence of soft drusen using an integrated experimental and modeling approach. We collect RPE flat mount images from donated human eyes and develop 1) statistical tools to quantify the images and 2) a cell-based model to simulate the morphology evolution. We compare three different mechanisms of RPE repair evolution, cell apoptosis, cell fusion, and expansion, and Simulations of our RPE morphogenesis model quantitatively reproduce deformations of human RPE morphology due to drusen, suggesting that a purse-string mechanism is sufficient to explain how RPE heals cell loss caused by drusen-damage. We found that drusen beneath tissue promote cell death in a number that far exceeds the cell numbers covering the drusen. Tissue deformations are studied using area distributions, Voronoi domains and a texture tensor.
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