大肠杆菌金属蛋白过表达的X射线显微断层显像
X-ray microtomographic visualization of Escherichia coli by metalloprotein overexpression
摘要：
本文报告了X射线显微断层显像的微生物大肠杆菌过表达金属蛋白铁蛋白。用同步辐射显微摄影仪和简单的投影几何结构测定了线性吸收系数的三维分布，结果表明X射线吸收是均匀分布的，表明每个E.用铁蛋白标记大肠杆菌细胞。表达铁蛋白的E.大肠杆菌显示出与磷钨酸染色的细胞相当的线性吸收系数。表达铁蛋白的E.使用配备有菲涅耳波带片的成像显微断层摄影机，通过Zernike相差使大肠杆菌细胞可视化。所获得的图像显示对应于E. coli细胞。这些结果表明，金属蛋白的过表达促进生物物体的三维细胞结构的X射线可视化。
英文摘要：
This paper reports X-ray microtomographic visualization of the microorganism Escherichia coli overexpressing a metalloprotein ferritin. The three-dimensional distribution of linear absorption coefficients determined using a synchrotron radiation microtomograph with a simple projection geometry revealed that the X-ray absorption was homogeneously distributed, suggesting that every E. coli cell was labeled with the ferritin. The ferritin-expressing E. coli exhibited linear absorption coefficients comparable to those of phosphotungstic-acid stained cells. The submicrometer structure of the ferritin-expressing E. coli cells was visualized by Zernike phase contrast using an imaging microtomograph equipped with a Fresnel zone plate. The obtained images revealed curved columnar or bunching oval structures corresponding to the E. coli cells. These results indicate that the metalloprotein overexpression facilitates X-ray visualization of three-dimensional cellular structures of biological objects.
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