氟化表面：面向Laser脱附电离的通用无基质基底
Fluorinated surfaces: towards a universal matrix-free substrates for Laser Desorption Ionization
摘要：
基质辅助激光解吸电离（MALDI）是一种软电离方法，广泛应用于高通量质谱分析。这种技术的主要限制之一是它需要使用基质：这些分子使分析物能够解吸和电离（D/I）过程，但也在低质谱区域产生强干扰信号，从而阻止低分子量化合物的适当检测。避免使用它们的可能性将简化样品制备和质谱（MS）解释。近年来，纳米结构表面已被提出作为一种可行的方法来实现这一目标，但结果仅限于特定类别的化合物和方法缺乏一般性。在这里，我们证明了表面的活化是激活目标和实现无基质操作所需的唯一步骤，使用纳米结构芯片获得相同的检测限。这项工作的结果表明，D/I是一个纯粹的界面效应，与下面的基板的化学性质的贡献可以忽略不计，只要基板是导电的，并能够有效地吸收紫外激光束。
英文摘要：
Matrix Assisted Laser Desorption Ionization (MALDI) is a soft ionization method that finds widespread applications in high-throughput mass spectrometric analysis. One of the main limit of this technique is that it requires the use of matrices: these molecules enable the analytes desorption and ionization (D/I) processes but also generate strong interfering signals in the low mass spectral region, preventing a suitable detection of low molecular weight compounds. The possibility to avoid their use will ease both sample preparation and mass spectrum (MS) interpretation. In recent years nanostructured surfaces have been proposed as a viable method to achieve such goal but the results are limited to specific classes of chemical compounds and the approach lacks in generality. Here we demonstrate that the fluorination of surfaces is the only step needed to activate the target and to achieve matrix-free operation with the same detection limit obtained using nanostructured chips. The results of this work suggest that D/I is a purely interfacial effect, with negligible contribution from chemical nature of the underlying substrate, provided that the substrate is conductive and able to efficiently absorb the UV laser beam.
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