重组酶蛋白是一种扭矩敏感的分子开关
The recombinase protein is a torque sensitive molecular switch
摘要：
纳米粒子如何寻找纳米目标是非平衡统计物理中的一个普遍问题。当一个受损的DNA片段试图在完整的同源染色体上找到它的对应物时，它就变得至关重要。如果两个拷贝配对，完整的同源序列就可以作为模板来重建受损的DNA序列，使细胞能够在没有基因突变的情况下存活。要想成功，搜索必须在找到完美的同源性时才能停止，确保这种基因组完整性的生物学过程被称为同源重组，并由同源重组酶蛋白促进。在这篇文章中，我们使用扭矩敏感的磁镊测量的自由能景观的人端粒酶hRad 51蛋白组装的DNA片段。基于我们的测量，我们将hRad 51/DNA复合物建模为一个不平衡的两态系统，并提供了一个homomorphonation的数学描述。通过这个动态的两态模型，我们提出了一种机制，通过该机制，重组酶蛋白质区分同源和非同源序列。
英文摘要：
How a nano-searcher finds its nano-target is a general problem in non-equilibrium statistical physics. It becomes vital when the searcher is a damaged DNA fragment trying to find its counterpart on the intact homologous chromosome. If the two copies are paired, that intact homologous sequence serves as a template to reconstitute the damaged DNA sequence, enabling the cell to survive without genetic mutations. To succeed, the search must stop only when the perfect homology is found. The biological process that ensures such a genomic integrity is called Homologous Recombination and is promoted by the Recombinase proteins. In this article, we use torque-sensitive magnetic tweezers to measure the free-energy landscape of the human Recombinase hRad51 protein assembled a DNA fragment. Based on our measurements we model the hRad51/DNA complex as an out-of-equilibrium two-state system and provide a thermodynamical description of Homologous Recombination. With this dynamical two-state model, we suggest a mechanism by which the recombinase proteins discriminate between homologous and a non-homologous sequences.
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