用于细胞测定的动态生化梯度的产生和精确控制
Generation and precise control of dynamic biochemical gradients for cellular assays
摘要：
可扩散信号分子的空间梯度在控制不同的细胞行为如细胞分化、组织图案化和趋化性中起着至关重要的作用。在本文中，我们报告的设计和测试的微流控装置的扩散为基础的梯度生成细胞检测。该器件独特的通道设计消除了源通道和汇通道之间的交叉流动，从而通过被动扩散稳定梯度。该平台还可以快速灵活地控制化学品浓度，从而在扩散室中形成高度动态的梯度。扩散和表面吸附的分子的第一近似模型概括了实验观察到的梯度。在化学诱导剂的梯度中培养的芽殖酵母细胞以浓度依赖性方式表达报告荧光蛋白。这种微流体平台作为一个多功能的原型适用于广泛的生物医学研究。
英文摘要：
Spatial gradients of diffusible signalling molecules play crucial roles in controlling diverse cellular behaviour such as cell differentiation, tissue patterning and chemotaxis. In this paper, we report the design and testing of a microfluidic device for diffusion-based gradient generation for cellular assays. A unique channel design of the device eliminates cross-flow between the source and sink channels, thereby stabilising gradients by passive diffusion. The platform also enables quick and flexible control of chemical concentration that makes highly dynamic gradients in diffusion chambers. A model with the first approximation of diffusion and surface adsorption of molecules recapitulates the experimentally observed gradients. Budding yeast cells cultured in a gradient of a chemical inducer expressed a reporter fluorescence protein in a concentration-dependent manner. This microfluidic platform serves as a versatile prototype applicable to a broad range of biomedical investigations.
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