静电纺聚ε-己内酯纳/微结构及自组装肽纤维的研究
An investigation into the nano-/micro-architecture of electrospun poly (ε-caprolactone) and self-assembling peptide fibers
摘要：
自组装肽（SAP）具有自发组装成有序纳米结构的能力，从而能够制造“设计师”纳米材料。SAP的可逆分子缔合已被证明在治疗学中提供了巨大的希望，例如，通过设计用于硬组织再生的仿生组件。这可以进一步用于新型纳米/微米诊断工具。然而，自组装肽凝胶通常与固有的弱的和瞬时的机械性能相关联。将其掺入聚合物基质中被认为是提高其机械稳定性的潜在策略。本研究的重点是将11个残基的肽P11 - 8（肽序列：CH 3CO-Gln-Gln-Arg-Phe-Orn-Trp-Orn-Phe-Glu-Gln-Gln-NH2）掺入聚（ε-己内酯）（PCL）纤维支架中。在这项研究中，使用静电纺丝技术从P11 - 8和PCL的溶液中制备仿生多孔支架，其导致由亚微米纤维（直径为100 - 700 nm）和纳米纤维（直径为10 - 100 nm）组成的两相结构。织物的内部形态及其微观结构可以通过改变肽浓度来容易地控制。用ATR-FTIR光谱研究了初生纤维中P11 - 8的二级构象，结果表明，在纤维形成和纤维网沉积过程中，P11 - 8发生了自组装成β-片带的过程.
英文摘要：
Self-assembling peptides (SAPs) have the ability to spontaneously assemble into ordered nanostructures enabling the manufacture of "designer" nanomaterials. The reversible molecular association of SAPs has been shown to offer great promise in therapeutics via for example, the design of biomimetic assemblies for hard tissue regeneration. This could be further exploited for novel nano/micro diagnostic tools. However, self-assembled peptide gels are often associated with inherent weak and transient mechanical properties. Their incorporation into polymeric matrices has been considered as a potential strategy to enhance their mechanical stability. This study focuses on the incorporation of an 11-residue peptide, P11-8 (peptide sequence: CH3CO-Gln-Gln-Arg-Phe-Orn-Trp-Orn-Phe-Glu-Gln-Gln-NH2) within a fibrous scaffold of poly (ε-caprolactone) (PCL). In this study an electrospinning technique was used to fabricate a biomimetic porous scaffold out of a solution of P11-8 and PCL which resulted in a biphasic structure composed of submicron fibers (diameter of 100-700 nm) and nanofibers (diameter of 10-100 nm). The internal morphology of the fabric and its micro-structure can be easily controlled by changing the peptide concentration. The secondary conformation of P11-8 was investigated in the as-spun fibers by ATR-FTIR spectroscopy and it is shown that peptide self-assembly into \{b}eta-sheet tapes has taken place during fiber formation and the deposition of the fibrous web.
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