两种不同分子量胶原多肽的可纺性及交联纤维性能
Wet-spinnability and crosslinked fibre properties of two collagen polypeptides with varied molecular weight
摘要：
为了在分子和微观尺度上模拟组织的特征，形成具有湿稳定纤维结构的天然来源的材料是至关重要的。在这里，我们研究了基于胶原蛋白衍生的多肽的湿纺纤维的形成，所述胶原蛋白衍生的多肽具有可比的化学组成和不同的分子量。明胶和水解鱼胶原蛋白（HFC）被选为广泛使用的线性氨基酸链的高分子量和低分子量，分别和功能化的湿纺纤维状态，以保持在生理条件下的材料几何形状。湿纺纤维的直径和形态显着影响取决于多肽的分子量，湿纺溶剂（即2，2，2-三氟乙醇和二甲亚砜）和纺丝介质（即丙酮和乙醇），导致在庞大的或多孔的内部几何形状。与通过分子内马来酰亚胺介导的缩合反应交联的样品（E：588 +/- 38 MPa）相比，在与活化的1，3-苯二乙酸（Ph）共价交联后，明胶和HFC样品的干态拉伸模量显著增强（E：726 +/- 43 - 844 +/- 85 MPa）。所得纤维显示出在238 +/-18-355 +/-28微米范围内的干直径，并且证明在用PBS平衡后是机械稳定的（E：230 kPa），同时在生理条件下孵育5天后观察到几乎完全降解。
英文摘要：
The formation of naturally-derived materials with wet stable fibrous architectures is paramount in order to mimic the features of tissues at the molecular and microscopic scale. Here, we investigated the formation of wet-spun fibres based on collagen-derived polypeptides with comparable chemical composition and varied molecular weight. Gelatin and hydrolysed fish collagen (HFC) were selected as widely-available linear amino-acidic chains of high and low molecular weight, respectively, and functionalised in the wet-spun fibre state in order to preserve the material geometry in physiological conditions. Wet-spun fibre diameter and morphology were dramatically affected depending on the polypeptide molecular weight, wet-spinning solvent (i.e. 2,2,2-Trifluoroethanol and dimethyl sulfoxide) and coagulating medium (i.e. acetone and ethanol), resulting in either bulky or porous internal geometry. Dry-state tensile moduli were significantly enhanced in gelatin and HFC samples following covalent crosslinking with activated 1,3 phenylenediacetic acid (Ph) (E: 726 +/- 43 - 844 +/- 85 MPa), compared to samples crosslinked via intramolecular carbodiimide-mediated condensation reaction (E: 588 +/- 38 MPa). Resulting fibres displayed a dry diameter in the range of 238 +/- 18 - 355 +/- 28 micron and proved to be mechanically-stable (E: 230 kPa) following equilibration with PBS, whilst a nearly-complete degradation was observed after 5-day incubation in physiological conditions.
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