高能质子束对大肠杆菌作用的研究
Study of the Effects of High-Energy Proton Beams on Escherichia Coli
摘要：
抗生素耐药细菌感染成为当今公共卫生保健最严重的风险之一。然而，令人沮丧的是，在过去十年中，新抗生素的开发进展甚微。因此，迫切需要寻找治疗耐药菌的替代方法。新的方法，其中包括光热疗法的基础上的金纳米材料和电离辐射，如X射线和γ射线，已被报道。高能质子辐射对细菌的影响研究主要集中在芽孢杆菌及其芽孢上。研究了质子束对大肠杆菌（Escherichia coli，E.大肠杆菌）的报道有限。大肠杆菌是获取代谢和遗传信息的重要生物学工具，也是研究毒性和抗菌活性的常用模式微生物。此外，E.大肠杆菌是哺乳动物肠道内常见的细菌。本文对E.大肠杆菌的质子辐照。细胞的稀释溶液用于质子束辐射。LB琼脂平板用于计数形成的菌落数。通过600 nm处的光密度监测细胞的生长曲线。光学显微镜下观察细胞形态学变化。微阵列分析检测辐照样品和未辐照对照样品之间的基因表达变化。
英文摘要：
Antibiotic-resistant bacterial infection becomes one of the most serious risks to public health care today. However, discouragingly, the development of new antibiotics has been little progressed over the last decade. There is an urgent need of the alternative approaches to treat the antibiotic-resistant bacteria. The novel methods, which include photothermal therapy based on gold nano-materials and ionizing radiation such as X-rays and gamma rays, have been reported. Studies of the effects of high-energy proton radiation on bacteria are mainly focused on Bacillus species and its spores. The effect of proton beams on Escherichia coli (E. coli) has been limitedly reported. The Escherichia coli is an important biological tool to obtain the metabolic and genetic information and also a common model microorganism for studying toxicity and antimicrobial activity. In addition, E. coli is a common bacterium in the intestinal tract of mammals. Herein, the morphological and physiological changes of E. coli after proton irradiation were investigated. The diluted solutions of the cells were used for proton beam radiation. LB agar plates were used to count the number of colonies formed. The growing profile of the cells was monitored by optical density at 600 nm. The morphology of the irradiated cells was analyzed with optical microscope. Microarray analysis was performed to examine the gene expression changes between irradiated samples and control samples without irradiation.
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