启动子活性与其间隔区核苷酸位置的相关性
Correlations between promoter activity and its nucleotide positions in spacing region
摘要：
转录是细胞读取基因编码的遗传信息的重要过程之一，转录是由RNA聚合酶与相关启动子结合而启动的。实验发现，启动子的核苷酸序列对基因表达强度，或启动子活性有很大影响。在合成生物学中，一个有趣的问题是我们如何合成具有给定活性的启动子，以及启动子序列的哪些位置对于决定其活性是重要的。在这项研究中，根据最近的实验数据，启动子活性和它的序列位置之间的相关性进行了分析，通过各种方法。我们的结果表明，除了两个高度保守区域$-35$ box和$-10$ box中的核苷酸外，其他位置的核苷酸的影响也是不可忽略的。例如，间隔区中位置$-19$周围的核苷酸的修饰可以大规模地改变启动子活性。本研究结果有助于我们理解基因转录的生物物理机制，也有助于设计合成细胞工厂。
英文摘要：
Transcription is one of the essential processes for cells to read genetic information encoded in genes, which is initiated by the binding of RNA polymerase to related promoter. Experiments have found that the nucleotide sequence of promoter has great influence on gene expression strength, or promoter activity. In synthetic biology, one interesting question is how we can synthesize a promoter with given activity, and which positions of promoter sequence are important for determining its activity. In this study, based on recent experimental data, correlations between promoter activity and its sequence positions are analyzed by various methods. Our results show that, except nucleotides in the two highly conserved regions, $-35$ box and $-10$ box, influences of nucleotides in other positions are also not neglectable. For example, modifications of nucleotides around position $-19$ in spacing region may change promoter activity in a large scale. The results of this study might be helpful to our understanding of biophysical mechanism of gene transcription, and may also be helpful to the design of synthetic cell factory.
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