化学气相沉积法在铜纳米颗粒上直接生长石墨烯作为腺苷的表面增强拉曼散射无标记检测
Direct Growth Graphene on Cu Nanoparticles by Chemical Vapor Deposition as Surface-Enhanced Raman Scattering Substrate for Label-Free Detection of Adenosine
摘要：
我们提出了一种石墨烯/铜纳米粒子杂化物（G/CuNPs）系统作为腺苷检测的表面增强拉曼散射（SERS）基底。在甲烷和氢气的混合气体中，通过化学气相沉积法在平板石英上直接合成了包裹在单层石墨烯壳上的铜纳米颗粒。G/CuNPs对腺苷具有很好的SERS增强活性。证明血清中腺苷的最小检测浓度低至5 nM，校准曲线在5至500 nM范围内显示出良好的线性响应。研究了G/CuNPs对正常人尿样中腺苷的SERS检测能力，发现腺苷的特征峰仍能被识别。可再现的和超灵敏的增强拉曼信号可能是由于石墨烯层的存在。石墨烯壳能够富集和固定腺苷分子，这也可以有效地保持G/CuNPs的化学和光学稳定性。基于G/CuNPs体系，腺苷在不同基质中的超灵敏SERS检测有望在医学和生物技术领域得到实际应用
英文摘要：
We present a graphene/Cu nanoparticle hybrids (G/CuNPs) system as a surface-enhanced Raman scattering (SERS) substrate for adenosine detection. The Cu nanoparticles wrapped around a monolayer graphene shell were directly synthesized on flat quartz by chemical vapor deposition in a mixture of methane and hydrogen. The G/CuNPs showed an excellent SERS enhancement activity for adenosine. The minimum detected concentration of the adenosine in serum was demonstrated as low as 5 nM, and the calibration curve showed a good linear response from 5 to 500 nM. The capability of SERS detection of adenosine in real normal human urine samples based on G/CuNPs was also investigated and the characteristic peaks of adenosine were still recognizable. The reproducible and the ultrasensitive enhanced Raman signals could be due to the presence of an ultrathin graphene layer. The graphene shell was able to enrich and fix the adenosine molecules, which could also efficiently maintain chemical and optical stability of G/CuNPs. Based on G/CuNPs system, the ultrasensitive SERS detection of adenosine in varied matrices was expected for the practical applications of medicine and biotechnology
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