亚30 nm纳米缝中部分伸展的DNA蠕动的定量分析
Quantitative analysis of reptation of partially extended DNA in sub-30 nm nanoslits
摘要：
我们观察到单个DNA分子在高度低于30 nm的熔融石英纳米缝中的蠕动。利用取向相关和横向涨落分析定量地表征了蠕动行为和约束效应。我们表明，在强准二维限制和与纳米缝中的表面钝化聚乙烯吡咯烷酮（PVP）分子的相互作用下，紧张的聚合物会出现管状聚合物运动，而蚀刻诱导的器件表面粗糙度，芯片键合材料和DNA嵌入染料表面相互作用则起次要作用。这些发现具有强烈的影响，在纳米流体系统的表面改性的效果与单分子DNA分析的潜在应用。
英文摘要：
We observed reptation of single DNA molecules in fused silica nanoslits of sub-30 nm height. The reptation behavior and the effect of confinement are quantitatively characterized using orientation correlation and transverse fluctuation analysis. We show tube-like polymer motion arises for a tense polymer under strong quasi-2D confinement and interaction with surface- passivating polyvinylpyrrolidone (PVP) molecules in nanoslits, while etching- induced device surface roughness, chip bonding materials and DNA-intercalated dye-surface interaction, play minor roles. These findings have strong implications for the effect of surface modification in nanofluidic systems with potential applications for single molecule DNA analysis.
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