光学显微镜：一场正在进行的当代革命
Light Microscopy: An ongoing contemporary revolution
摘要：
光学显微镜是仍然用于前沿研究的最古老的科学仪器之一。Ernst Abbe在19世纪对显微镜分辨率极限的阐述让一代又一代的科学家相信，对单个分子的光学研究和分辨亚波长结构是不可行的。2014年诺贝尔奖的超分辨率荧光显微镜标志着一个明确的认识，即旧的信念必须重新审视。在这篇文章中，我们提出了一个批判性的概述各种最近的发展，光学显微镜。除了流行的超分辨率荧光方法，我们还讨论了各种其他技术和成像对比度的前景，并考虑了未来的一些基本和实际挑战。
英文摘要：
Optical microscopy is one of the oldest scientific instruments that is still used in forefront research. Ernst Abbe's nineteenth century formulation of the resolution limit in microscopy let generations of scientists believe that optical studies of individual molecules and resolving sub-wavelength structures were not feasible. The Nobel Prize in 2014 for super-resolution fluorescence microscopy marks a clear recognition that the old beliefs have to be revisited. In this article, we present a critical overview of various recent developments in optical microscopy. In addition to the popular super-resolution fluorescence methods, we discuss the prospects of various other techniques and imaging contrasts and consider some of the fundamental and practical challenges that lie ahead.
机构、总结、中文摘要均由AI分析而来，可能存在错误，请以论文正文为准。
