DNA发夹主要促进双链体解链而不是抑制杂交
DNA hairpins primarily promote duplex melting rather than inhibiting hybridization
摘要：
二级结构对DNA双链体形成的影响知之甚少。我们使用DNA的粗粒度模型表明，特定的3个和4个碱基对发夹结构分别将杂交率降低了2倍和10倍，与实验完全一致。相比之下，熔化速率加快了约100和约2000倍。这种令人惊讶的大加速发生，因为发夹形成在熔化过程中，稳定部分熔化状态，并促进解离。这些结果可能有助于指导DNA装置的设计，使用发夹来调节杂交和解离途径和速率。
英文摘要：
The effect of secondary structure on DNA duplex formation is poorly understood. We use a coarse-grained model of DNA to show that specific 3- and 4-base pair hairpins reduce hybridization rates by factors of 2 and 10 respectively, in good agreement with experiment. By contrast, melting rates are accelerated by factors of ~100 and ~2000. This surprisingly large speed-up occurs because hairpins form during the melting process, stabilizing partially melted states, and facilitating dissociation. These results may help guide the design of DNA devices that use hairpins to modulate hybridization and dissociation pathways and rates.
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