核心核糖体蛋白质之间的差异化学计量
Differential stoichiometry among core ribosomal proteins
摘要：
了解核糖体的调节和结构对于了解疾病中蛋白质合成及其失调至关重要。虽然核糖体被认为在其核心核糖体蛋白（RP）中具有固定的化学计量，但一些实验表明其组成更可变。测试这种变异性需要直接和精确的RP定量。我们使用质谱法直接定量小鼠胚胎干细胞（ESC）和芽殖酵母的单体和多聚体中的RP。我们的数据表明，在野生型酵母细胞和ESC的核心RP之间的化学计量依赖于生长条件和每个mRNA结合的核糖体的数量。此外，我们发现，与删除RP-基因的细胞的健身成反比，相应的RP在多聚核糖体的富集。总之，我们的研究结果支持存在具有不同蛋白质组成和生理功能的核糖体。
英文摘要：
Understanding the regulation and structure of ribosomes is essential to understanding protein synthesis and its deregulation in disease. While ribosomes are believed to have a fixed stoichiometry among their core ribosomal proteins (RPs), some experiments suggest a more variable composition. Testing such variability requires direct and precise quantification of RPs. We used mass-spectrometry to directly quantify RPs across monosomes and polysomes of mouse embryonic stem cells (ESC) and budding yeast. Our data show that the stoichiometry among core RPs in wild-type yeast cells and ESC depends both on the growth conditions and on the number of ribosomes bound per mRNA. Furthermore, we find that the fitness of cells with a deleted RP-gene is inversely proportional to the enrichment of the corresponding RP in polysomes. Together, our findings support the existence of ribosomes with distinct protein composition and physiological function.
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