使用高分辨率1H和1H-13 C NMR光谱法表征腹膜透析流出物中的代谢物
Metabolite Characterization in Peritoneal Dialysis Effluent Using High-resolution 1H and 1H-13C NMR Spectroscopy
摘要：
腹膜透析（PD）流出物的代谢物分析可提供与长期PD治疗相关的并发症的发生和进展相关的信息。在这种情况下，使用高分辨率1H和1H-13 C NMR光谱对PD流出物样品的NMR可检测小代谢物进行表征。记录了终末期肾衰竭（ESRF）患者在4小时（腹膜内）停留时间后获得的PD流出液样本的各种光谱（在800 MHz质子频率下），并继续正常进行PD治疗。尽管由于存在来自葡萄糖和乳酸的强共振，PD流出物具有破坏性的光谱特征，但我们能够鉴定出约53种小的内源性代谢物（包括许多复杂的耦合自旋系统），并且1H-13 C HSQC光谱的总CH交叉峰中超过90%被鉴定为特定于PD流出物的各种代谢物。我们预见，对照PD流出液样本的各种代谢物的特征指纹将用于识别和区分PD相关并发症的代谢差异。
英文摘要：
Metabolite analysis of peritoneal dialysis (PD) effluent may provide information regarding onset and progression of complications associated with prolonged PD therapy. In this context, the NMR detectable small metabolites of PD effluent samples were characterized using high resolution 1H and 1H-13C NMR spectroscopy. The various spectra were recorded (at 800 MHz proton frequency) on PD effluent samples obtained after 4 hour (intraperitoneal) dwell time from patients with end stage renal failure (ESRF) and continuing normally on PD therapy. Inspite of devastating spectral feature of PD effluent due to the presence of intense resonances from glucose and lactate, we were able to identify about 53 small endogenous metabolites (including many complex coupled spin systems) and more than 90 % of the total CH cross peaks of 1H-13C HSQC spectrum were identified specific to various metabolites of PD effluent. We foresee that the characteristic fingerprints of various metabolites of control PD effluent samples will be used to identify and distinguish metabolic differences from PD related complications.
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