突破Crowther极限：结合深度切片和CT进行高分辨率、宽视场3D重建
Breaking the Crowther Limit: Combining Depth-Sectioning and Tilt Tomography for High-Resolution, Wide-Field 3D Reconstructions
摘要：
到目前为止，三维（3D）扩展对象的高分辨率（< 1 nm）成像还不可能。被称为Crowther标准的限制迫使通过断层扫描进行3D重建的对象大小和分辨率之间的权衡。此外，像差校正电子显微镜的亚埃分辨率伴随着景深的极大减小，导致较大样本（> 6 nm）的区域看起来模糊或缺失。在这里，我们展示了一种三维成像方法，克服了这两个限制相结合，通过焦点深度切片和传统的倾斜系列断层扫描重建扩展对象，高分辨率，在所有三个维度。像差校正仪器中的大会聚角现在变成了一个优点，而不是更高质量重建的障碍。在含有超过一百个去合金化和纳米多孔PtCu催化剂颗粒的390 nm 3D碳载体上的穿透焦点重建以亚纳米细节揭示了由去合金化不稳定性产生的广泛和连接的内部孔结构。
英文摘要：
To date, high-resolution (< 1 nm) imaging of extended objects in three-dimensions (3D) has not been possible. A restriction known as the Crowther criterion forces a tradeoff between object size and resolution for 3D reconstructions by tomography. Further, the sub-Angstrom resolution of aberration-corrected electron microscopes is accompanied by a greatly diminished depth of field, causing regions of larger specimens (> 6 nm) to appear blurred or missing. Here we demonstrate a three-dimensional imaging method that overcomes both these limits by combining through-focal depth sectioning and traditional tilt-series tomography to reconstruct extended objects, with high-resolution, in all three dimensions. The large convergence angle in aberration corrected instruments now becomes a benefit and not a hindrance to higher quality reconstructions. A through-focal reconstruction over a 390 nm 3D carbon support containing over one hundred dealloyed and nanoporous PtCu catalyst particles revealed with sub-nanometer detail the extensive and connected interior pore structure that is created by the dealloying instability.
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