变形细胞迁移过程中牵引力的分布及与形态变化相关的细胞内标志物
Distribution of Traction Forces and Intracellular Markers Associated with Shape Changes During Amoeboid Cell Migration
摘要：
在迁移过程中，变形虫细胞执行准周期性重复事件的周期（运动周期）。单元长度和与衬底交换的应变能以平均频率f随时间振荡，在平均频率f之上施加较小的随机波动。细胞形状的相当大一部分变化是由于周期性重复事件引起的，这一事实使得能够使用条件统计方法来分析涉及细胞运动性的生化过程网络。利用这种循环性质，我们应用主成分分析（PCa）和相平均统计来分析形状变化的主导模式及其与分子马达的活性和定位的关联。我们分析了延时测量的细胞形状，牵引力和荧光从绿色荧光蛋白（GfP）报告的f-肌动蛋白在Dictyosteoblastoma细胞进行引导趋化迁移。以野生型细胞（WT）为参照，通过研究肌球蛋白II重链缺失突变细胞（mhcA-）和肌球蛋白II必需轻链缺失细胞（mlcE-），我们研究了肌球蛋白II的收缩和肌动蛋白交联功能。
英文摘要：
During migration, amoeboid cells perform a cycle of quasi-periodic repetitive events (motility cycle). the cell length and the strain energy exchanged with the substrate oscillate in time with an average frequency, f, on top of which are imposed smaller random fluctuations. the fact that a considerable portion of the changes in cell shape are due to periodic repetitive events enables the use of conditional statistics methods to analyze the network of biochemical processes involved in cell motility. taking advan- tage of this cyclic nature, we apply Principal Component analysis (PCa) and phase- average statistics to analyze the dominant modes of shape change and their association to the activity and localization of molecular motors. We analyze time-lapse measure- ments of cell shape, traction forces and fluorescence from green fluorescent protein (GfP) reporters for f-actin in Dictyostelium cells undergoing guided chemotactic migration. using wild-type cells (wt) as reference, we investigated the contractile and actin crosslinking functions of myosin II by studying myosin II heavy chain null mutant cells (mhcA-) and myosin II essential light chain null cells (mlcE-).
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