核SSR位点的保守性揭示了没食子栎亚种的高亲和力。veneris A. Kern（壳斗科）至Robur组
Conservation of nuclear SSR loci reveals high affinity of Quercus infectoria ssp. veneris A. Kern (Fagaceae) to section Robur
摘要：
对16个核微卫星位点进行了保守性研究，这些位点最初是为大果栎（Quercus macrocarpa）（高山栎组）、高山栎（Quercus macrocarpa）、高山栎（Quercus macrocarpa）和高山栎（Quercus macrocarpa）开发的。petraea，Q. robur（Robur组）和Q. myrsinifolia，青冈亚属（Cyclobalanopsis）的青冈（Q.没食子草属来自塞浦路斯的veneris种群。所有的基因座都可以成功地扩增，并显示等位基因的大小和多样性模式，符合那些属于部分罗布的橡树种。至少在一种情况下，有限的扩增和高水平的纯合性支持“无效等位基因”的发生，这是由高度保守的引物区域中的可能突变引起的，从而阻碍了PCR。采样的人口表现出高水平的多样性，尽管该物种在塞浦路斯的分布非常有限，并扩大人口碎片。Q.在QpZAG9位点上与无茵茨孢属的部分序列相匹配。Alnifolia和Q.来自邻近种群的球虫。然而，测序显示同源性，不包括种间渗入的情况下，与后者，遗传学上遥远的物种。Q.没食子草属veneris在该位点的序列与Robur组的其它物种一致，而Quercus alnifolia和Quercus coccifera与Q.塞里斯。
英文摘要：
Conservation of 16 nuclear microsatellite loci, originally developed for Quercus macrocarpa (section Albae), Q. petraea, Q. robur (section Robur) and Q. myrsinifolia, (subgenus Cyclobalanopsis) was tested in a Q. infectoria ssp. veneris population from Cyprus. All loci could be amplified successfully and displayed allele size and diversity patterns that match those of oak species belonging to the section Robur. At least in one case, limited amplification and high levels of homozygosity support the occurrence of 'null alleles', caused by a possible mutation in the highly conserved primer areas, thus hindering PCR. The sampled population exhibited high levels of diversity despite the very limited distribution of this species in Cyprus and extended population fragmentation. Allele sizes of Q. infectoria at locus QpZAG9 partially match those of Q. alnifolia and Q. coccifera from neighboring populations. However, sequencing showed homoplasy, excluding a case of interspecific introgression with the latter, phylogenetically remote species. Q. infectoria ssp. veneris sequences at this locus were concordant to those of other species of section Robur, while sequences of Quercus alnifolia and Quercus coccifera were almost identical to Q. cerris.
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