阻断离子电流的涨落揭示石墨烯纳米孔中DNA的瞬时状态
The Fluctuations of Blocked Ionic Current Reveal the Instantaneous Statuses of DNA in Graphene Nanopore
摘要：
从被阻断的离子电流中提取DNA序列信息是基于离子电流的纳米孔测序方法的关键步骤。最薄的石墨烯纳米孔仅包含一层碳原子，可能具有超高的DNA测序灵敏度。然而，迄今为止，被阻断的离子电流中所包含的DNA的动力学易位信息还没有被很好地理解。本文采用分子动力学模拟对基于离子电流的石墨烯纳米孔DNA传感方法的灵敏度进行了评估。通过滤除分子热运动引起的离子电流噪声，我们发现去噪后的离子电流波动可以揭示石墨烯纳米孔中DNA的瞬时构象变化。然而，也观察到由DNA碱基对与纳米孔的接近（在1.5nm内）引起的离子电流的阻断。虽然石墨烯纳米孔的预期单碱基分辨率还有待进一步研究，但我们的研究结果表明，基于离子电流的石墨烯纳米孔传感方法对DNA的瞬时易位状态具有高灵敏度。
英文摘要：
Extracting the sequence information of DNA from the blocked ionic current is the crucial step of the ionic current-based nanopore sequencing approaches. The thinnest graphene nanopore, which contained only one layer of carbon atoms, potentially has ultra-high DNA sequencing sensitivity. However, the dynamical translocation information of DNA contained in the blocked ionic current has not been well understood to date. In this letter, an assessment to the sensitivity of ionic current-based graphene nanopore DNA sensing approach was carried out using molecular dynamics simulations. By filtering the molecular thermal motion induced noise of ionic current, we found that the instantaneous conformational variations of DNA in graphene nanopore could be revealed from the fluctuations of the denoised ionic current. However, the blockage of ionic current which induced by the proximity of the DNA base-pairs to the nanopore (within 1.5 nm) was also observed. Although the expected single-base resolution of graphene nanopore should be enhanced by further studies, our findings indicated that the ionic current-based graphene nanopore sensing approach has high sensitivity to the instantaneous translocation status of DNA.
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