真菌系统学中的分子数据分析：有抱负的研究人员指南
Incorporating molecular data in fungal systematics: a guide for aspiring researchers
摘要：
在过去的二十年里，分子数据已经成为真菌学大多数分支的主要研究工具。真菌系统学、分类学和生态学都取得了巨大的进步，并随着DNA测序技术的不断改进而发生了迅速而深远的变化。利用分子数据进行的分类学研究涉及一系列步骤，从分类群取样到各种实验室程序和数据分析，再到出版过程。所有步骤都很重要，影响结果和科学界对结果的看法。本文件提供了一个反思性的概述，在这样一个项目的目的，以帮助研究学生即将开始他们的第一次学习使用基于DNA的方法的所有主要步骤。我们也借此机会讨论分类学在生物学和生命科学中的作用。虽然学习分子方法的最佳方式是与有经验的人并肩工作，但我们希望本文将有助于降低读者的学习门槛。
英文摘要：
The last twenty years have witnessed molecular data emerge as a primary research instrument in most branches of mycology. Fungal systematics, taxonomy, and ecology have all seen tremendous progress and have undergone rapid, far-reaching changes as disciplines in the wake of continual improvement in DNA sequencing technology. A taxonomic study that draws from molecular data involves a long series of steps, ranging from taxon sampling through the various laboratory procedures and data analysis to the publication process. All steps are important and influence the results and the way they are perceived by the scientific community. The present paper provides a reflective overview of all major steps in such a project with the purpose to assist research students about to begin their first study using DNA-based methods. We also take the opportunity to discuss the role of taxonomy in biology and the life sciences in general in the light of molecular data. While the best way to learn molecular methods is to work side by side with someone experienced, we hope that the present paper will serve to lower the learning threshold for the reader.
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