走向实验室中古代历史的重演：结合合成生物学与实验进化
Towards the Recapitulation of Ancient History in the Laboratory: Combining Synthetic Biology with Experimental Evolution
摘要：
理解历史在进化轨迹中所起作用的一种方法是给古代生命第二次进化的机会。然而，我们凭经验进行这种实验的能力受到当前实验设计的限制。将祖先序列重建与合成生物学相结合，使我们能够在现代背景下恢复过去，并扩大了我们对历史背景下蛋白质功能的理解。另一方面，实验进化使我们能够在实验室的受控条件下研究进化。在这里，我们描述了一种新的实验装置，集成了两个不同的领域-祖先序列重建和实验进化。这使我们能够在实验室中倒带和重放古代生物分子的进化历史。我们预计，我们的结合将提供一个更深入的理解的潜在作用，偶然性和决定论在塑造进化过程中发挥作用。
英文摘要：
One way to understand the role history plays on evolutionary trajectories is by giving ancient life a second opportunity to evolve. Our ability to empirically perform such an experiment, however, is limited by current experimental designs. Combining ancestral sequence reconstruction with synthetic biology allows us to resurrect the past within a modern context and has expanded our understanding of protein functionality within a historical context. Experimental evolution, on the other hand, provides us with the ability to study evolution in action, under controlled conditions in the laboratory. Here we describe a novel experimental setup that integrates two disparate fields - ancestral sequence reconstruction and experimental evolution. This allows us to rewind and replay the evolutionary history of ancient biomolecules in the laboratory. We anticipate that our combination will provide a deeper understanding of the underlying roles that contingency and determinism play in shaping evolutionary processes.
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