在E.大肠杆菌：一个随机聚合模型
Stuttering Min oscillations within E. coli bacteria: A stochastic polymerization model
摘要：
我们建立了一个三维非格子随机聚合模型来研究大肠杆菌中Min蛋白的亚细胞振荡，并用它来研究Min振荡口吃的实验现象.口吃的影响，立即重新绑定的速度释放的MinE解聚丝尖（持续合成能力），保护解聚丝尖从MinD绑定，和MinD丝由于MinE的碎片。每一个持续合成能力，保护，和碎片减少口吃，速度振荡，并减少MinD细丝长度。无论是持续性还是尖端保护，本身都不足以产生快速的无口吃振荡。虽然细丝碎片本身可能导致快速振荡，但不经常出现口吃;高水平的碎片会降低振荡。在标准Min振荡中观察到的罕见口吃与MinD的短细丝一致，而我们预计表现出更高口吃频率的突变体将表现出更长的MinD细丝。增加的口吃率可能是一个有用的诊断，发现可观察到的MinD聚合在实验条件下。
英文摘要：
We have developed a 3D off-lattice stochastic polymerization model to study subcellular oscillation of Min proteins in the bacteria Escherichia coli, and used it to investigate the experimental phenomenon of Min oscillation stuttering. Stuttering was affected by the rate of immediate rebinding of MinE released from depolymerizing filament tips (processivity), protection of depolymerizing filament tips from MinD binding, and fragmentation of MinD filaments due to MinE. Each of processivity, protection, and fragmentation reduces stuttering, speeds oscillations, and reduces MinD filament lengths. Neither processivity or tip-protection were, on their own, sufficient to produce fast stutter-free oscillations. While filament fragmentation could, on its own, lead to fast oscillations with infrequent stuttering; high levels of fragmentation degraded oscillations. The infrequent stuttering observed in standard Min oscillations are consistent with short filaments of MinD, while we expect that mutants that exhibit higher stuttering frequencies will exhibit longer MinD filaments. Increased stuttering rate may be a useful diagnostic to find observable MinD polymerization in experimental conditions.
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