Long-Evans大鼠背外侧膝状体亚结构：霍乱毒素B亚单位的研究
Dorsal lateral geniculate substructure in the Long-Evans rat: A cholera toxin B-subunit study
摘要：
本研究描述了基于视网膜神经节细胞输入的起源眼的有色大鼠（Rattus norvegicus）丘脑背外侧膝状体核的亚结构。我们单眼眼内注射霍乱毒素（CTB），一个敏感的顺行示踪剂的B亚单位，在三个成年雄性Long-Evans大鼠。在另外四名受试者中，我们在双眼中注射荧光团缀合的CTB，在每只眼睛中使用不同的荧光团。这些受试者的大脑被固定和切片，标记的视网膜神经节细胞末端用宽视场亚微米分辨率载玻片扫描仪成像。追踪视网膜终止区以重建大脑两侧dLGN中同侧和对侧视网膜终止区的三维模型。dLGN体积为1.58 \pm 0.094 mm^{3}，其中70 \pm占整个视网膜受体LGN体积的3%。我们发现视网膜终末被起源眼很好地分离。我们一直发现三个或四个空间上分离的同侧受体区内的每个dLGN，而不是单一的紧凑区预期。这些亚结构域是否代表不同的功能亚板层仍有待确定。
英文摘要：
This study describes the substructure of the dorsal lateral geniculate nucleus of the thalamus of the pigmented rat (Rattus norvegicus) based on the eye-of-origin of its retinal ganglion cell inputs. We made monocular intra-ocular injections of the B-subunit of cholera toxin (CTB), a sensitive anterograde tracer, in three adult male Long-Evans rats. In four additional subjects, we injected fluorophor-conjugated CTB in both eyes, using a different fluorophor in each eye. Brains of these subjects were fixed and sectioned, and the labeled retinal ganglion cell termini were imaged with wide-field sub-micron resolution slide scanners. Retinal termination zones were traced to reconstruct a three dimensional model of the ipsilateral and contralateral retinal termination zones in the dLGN on both sides of the brain. The dLGN volume was 1.58 \pm0.094 mm^{3}, comprising 70 \pm 3% the volume of the entire retinorecipient LGN. We find the retinal terminals to be well-segregated by eye of origin. We consistently found three or four spatially separated ipsilateral-recipient zones within each dLGN, rather than the single compact zone expected. It remains to be determined whether these subdomains represent distinct functional sublaminae.
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