TIRF显微镜观察表面纳米气泡成核
Surface Nanobubble Nucleation Visualized with TIRF Microscopy
摘要：
使用全内反射荧光（TIRF）激发用光学显微镜观察纳米气泡。我们报告了在5 $μ\,$ M浓度下使用罗丹明6 G的TIRF可视化，其结果是强烈对比的图片。优先吸收和高空间分辨率允许检测直径为230 nm及以上的纳米气泡。我们提出了一个研究的成核动力学从水-乙醇-水交换和报告的尺寸分布。在交换后4分钟内观察到纳米气泡成核，随后形成表面密度为0.55气泡/ $μ$ m $^2$的稳定纳米气泡群。有趣的是，在水-乙醇交换的第一阶段期间观察到不稳定的、缓慢溶解的纳米气泡;只有在乙醇-水交换之后才出现稳定的纳米气泡。
英文摘要：
Nanobubbles are observed with optical microscopy using the total internal reflection fluorescence (TIRF) excitation. We report on TIRF visualization using Rhodamine 6G at 5$μ\,$M concentration which results to strongly contrasted pictures. The preferential absorption and the high spatial resolution allow to detect nanobubbles with diameters of 230\,nm and above. We present a study of the nucleation dynamics from the water-ethanol-water exchange and report the size distributions. Nanobubble nucleation is observed within 4 min after the exchange, later a stable population of nanobubbles with a surface density of 0.55 bubbles\,/$μ$m$^2$ is formed. Interestingly, unstable, slowly dissolving nanobubbles are observed during the first stage of water-ethanol exchange; only after the ethanol-water exchange stable nanobubbles appear.
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