电渗透和基因电转移对GPi锚定蛋白侧向移动性的影响
Effects of electropermeabilization and gene electrotransfert on the lateral mobility of a GPi anchored protein
摘要：
电透化是一种通过质膜将分子引入活细胞的方法。在这里，我们表明，电渗透影响的侧向流动性的Rae-1，GPi锚定蛋白。我们的结果表明，10至20%的膜表面被缺陷或孔隙占据。基因电转移还影响Rae-1的侧向移动性。此外，我们清楚地表明，一旦插入质膜，DNA totaly排除Rae-1，表明DNA分子紧密地堆积在一起，形成巨大的聚集体。
英文摘要：
Electropermeabilization is a method by which molecules can be introduced in living cells through the plasma membrane. Here, we show that electropermeabilization affects the lateral mobility of Rae-1, a GPi anchored protein. Our results shows that 10 to 20 % of the membrane surface is occupied by defaults or pores. Gene electrotransfert also affects the lateral mobility of Rae-1. Furthermore, we clearly show that once inserted into the plasma membrane, DNA totaly excludes Rae-1, indicating that DNA molecules are tightly packed together to forms huge aggregates.
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