用于单细胞研究的温度响应微图案化基底
Thermoresponsive micropatterned substrates for single cell studies
摘要：
我们描述了设计的微图案化的表面单细胞研究，基于温敏聚合物刷。我们表明，刷制成的聚（N-异丙基丙烯酰胺）接枝在高表面密度显示优异的蛋白质和细胞的抗粘附性能。这样的刷子很容易通过深紫外光刻法在微米级图案化。适当选择的粘合剂图案形状，结合PNIPAM的温度依赖性溶胀特性，使我们能够使用聚合物刷作为微致动器，当温度降低到32摄氏度以下时，诱导细胞分离。
英文摘要：
We describe the design of micropatterned surfaces for single cell studies, based on thermoresponsive polymer brushes. We show that brushes made of poly(N-isopropylacrylamide) grafted at high surface density display excellent protein and cell anti-adhesive properties. Such brushes are readily patterned at the micron scale via deep UV photolithography. A proper choice of the adhesive pattern shapes, combined with the temperature-dependent swelling properties of PNIPAM, allow us to use the polymer brush as a microactuator which induces cell detachment when the temperature is reduced below 32degC.
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