更多发霉的数据:另一种支原体基因跨越硅屏障进入人类基因组
More Mouldy Data: Another mycoplasma gene jumps the silicon barrier into the human genome
摘要:
人类基因组序列数据库包含与霉菌支原体非常相似的DNA序列。看来这种霉菌不仅感染分子生物学实验室,而且被实验人员从污染的样本中提取出来,并插入基因库,就像它们是人类一样。至少有一个发霉的EST(表达序列标签)已从公共数据库转移到商业工具(Affyphidogram HG-U133加2.0微阵列)。我们报告了第二个例子(DA 466599),并建议有必要清理基因组数据库,但担心目前的工具将不足以捕捉已经跳过硅屏障的基因。
英文摘要:
The human genome sequence database contains DNA sequences very like those of mycoplasma molds. It appears such moulds infect not only molecular Biology laboratories but were picked up by experimenters from contaminated samples and inserted into GenBank as if they were human. At least one mouldy EST (Expressed Sequence Tag) has transferred from public databases to commercial tools (Affymetrix HG-U133 plus 2.0 microarrays). We report a second example (DA466599) and suggest there is a need to clean up genomic databases but fear current tools will be inadequate to catch genes which have jumped the silicon barrier.
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